The 39-year-old, who has four gold medals and made her debut at the Sydney Games in 2000, had previously said she was reluctant to go because she would not be able to take three-year-old daughter Olympia with her.
Speaking at her pre-Wimbledon press conference, Williams said: ‘I’m actually not on the Olympic list- not that I’m aware of. If so, then I shouldn’t be on it.’
Serena Williams is the latest big-name player to announce she will not be travelling to Tokyo
She declined to explain her reasoning, adding: ‘There’s a lot of reasons that I made my Olympic decision. I don’t feel like going into them today. Maybe another day. Sorry.’
Williams joins Rafael Nadal and Dominic Thiem in deciding not to compete in Tokyo, while Roger Federer and Novak Djokovic have not yet committed to taking part.
More to follow